所有作者:郑煜煌 周华英 张春迎 李慧 谌资 刘猛
作者单位:中南大学湘雅二医院艾滋病研究室
论文摘要:Little sequence information is available for human immunodeficiency virus type 1(HIV-1) vpr genes of Chinese origin。 In our study, we analyzed HIV-1 vpr genes isolated from HIV-1 infected patients of South China。 Reverse transcription polymerase chain reaction (RT-PCR) and nested PCR was used to amplify HIV-1 vpr gene。 Using BLAST, Clustal W and Mega biological analysis software, we found vpr sequences mainly included subtypes as follows: CRF01-AE (51。63%), subtype C (24。84%), subtype B (17。65%), CRF03-AB (3。92%) and CRF08-BC (1。31%)。 The proportion of the glutamine (Q) is 84。3% in the 77th position of the 154 deduced amino acid sequences, which differ from overseas reports。
关键词: 多态性 VPR基因 艾滋病病毒
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