所有作者：寸韡 陈捷 张莹 刘龙丁 李琦涵
作者单位：中国医学科学院
论文摘要：Nuclear proteins often form punctiform structures， but the precise mechanism for this process is unknown。 As a preliminary study， we investigated the aggregation of an HSV-１ immediate-early protein， infected-cell protein ２２ (ICP２２)， in the nucleus by observing the localization of ICP２２-EGFP fusion protein。 Results showed that， in high-level expression conditions， ICP２２-EGFP gradually concentrates in the nucleus， persists throughout the cell cycle without disaggregation even in the cell division phase， and is finally distributed to daughter cells。 We subsequently constructed a mammalian cell expression system， which had tetracycline-dependent transcriptional regulators。 Consequently， the location of ICP２２-EGFP in the nucleus changed with distinct induction conditions。 This suggests that the cellular location of ICP２２ is also influenced by promoter regulation， in addition to its own structure。 Our findings provide new clues for the investigation of transcriptional regulation of viral genes。 In addition， the non-protease reporter system we constructed could be utilized to evaluate the role of internal ribosome entry sites (IRES) on transcriptional regulation。
关键词： Herpes Simplex Virus1 (HSV-1) ICP22 transcriptional regulation cellularlocalization nuclear functi
免费下载《I型单纯疱疹病毒立即早期蛋白ICP22的细胞定位分析》PDF全文（已停止下载）
本站“论文下载”文章收集整理于“中国科技论文在线”，由于各种原因，本站已暂停论文下载！请前往“中国科技论文在线http://www.paper.edu.cn/”免费下载！
指出，范文大全立刻进行改正或删除有关内容! 
当前位置：